| Chapter title |
Interfacing Biocompatible Reactions with Engineered Escherichia coli
|
|---|---|
| Chapter number | 27 |
| Book title |
Heterologous Gene Expression in E.coli
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6887-9_27 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6885-5, 978-1-4939-6887-9
|
| Authors |
Stephen Wallace, Emily P. Balskus |
| Editors |
Nicola A. Burgess-Brown |
| Abstract |
Biocompatible chemistry represents a new way of merging chemical and biological synthesis by interfacing nonenzymatic reactions with metabolic pathways. This approach can enable the production of nonnatural molecules directly from renewable starting materials via microbial fermentation. When developing a new biocompatible reaction certain criteria must be satisfied, i.e., the reaction must be (1) functional in aqueous growth media at ambient temperature and pH, (2) nontoxic to the producing microorganism, and (3) have negligible effects on the targeted metabolic pathway. This chapter provides a detailed outline of two biocompatible reaction procedures (hydrogenation and cyclopropanation), and describes some of the chemical and microbiological experiments and considerations required during biocompatible reaction development. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Unspecified | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 43% |
| Agricultural and Biological Sciences | 1 | 14% |
| Unspecified | 1 | 14% |
| Unknown | 2 | 29% |