Chapter title |
Generation of Recombinant N-Linked Glycoproteins in E. coli
|
---|---|
Chapter number | 15 |
Book title |
Heterologous Gene Expression in E.coli
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6887-9_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6885-5, 978-1-4939-6887-9
|
Authors |
Benjamin Strutton, Stephen R. P. Jaffé, Jagroop Pandhal, Phillip C. Wright, Strutton, Benjamin, Jaffé, Stephen R. P., Pandhal, Jagroop, Wright, Phillip C. |
Editors |
Nicola A. Burgess-Brown |
Abstract |
The production of N-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, Escherichia coli. This methods chapter will outline the components and procedures needed to produce N-linked glycoproteins in E. coli, utilizing Campylobacter jejuni glycosylation machinery, although other related genes can be used with minimal tweaks to this methodology. To ensure a successful outcome, various methods will be highlighted that can confirm glycoprotein production to a high degree of confidence, including the gold standard of mass spectrometry analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 17% |
Student > Bachelor | 2 | 17% |
Researcher | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Unknown | 6 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 3 | 25% |
Biochemistry, Genetics and Molecular Biology | 2 | 17% |
Agricultural and Biological Sciences | 1 | 8% |
Unknown | 6 | 50% |