Chapter title |
Protein Folding Using a Vortex Fluidic Device
|
---|---|
Chapter number | 13 |
Book title |
Heterologous Gene Expression in E.coli
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6887-9_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6885-5, 978-1-4939-6887-9
|
Authors |
Joshua Britton, Joshua N. Smith, Colin L. Raston, Gregory A. Weiss |
Editors |
Nicola A. Burgess-Brown |
Abstract |
Essentially all biochemistry and most molecular biology experiments require recombinant proteins. However, large, hydrophobic proteins typically aggregate into insoluble and misfolded species, and are directed into inclusion bodies. Current techniques to fold proteins recovered from inclusion bodies rely on denaturation followed by dialysis or rapid dilution. Such approaches can be time consuming, wasteful, and inefficient. Here, we describe rapid protein folding using a vortex fluidic device (VFD). This process uses mechanical energy introduced into thin films to rapidly and efficiently fold proteins. With the VFD in continuous flow mode, large volumes of protein solution can be processed per day with 100-fold reductions in both folding times and buffer volumes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 16% |
Student > Doctoral Student | 2 | 11% |
Student > Ph. D. Student | 2 | 11% |
Student > Bachelor | 2 | 11% |
Other | 1 | 5% |
Other | 3 | 16% |
Unknown | 6 | 32% |
Readers by discipline | Count | As % |
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Engineering | 2 | 11% |
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Unspecified | 1 | 5% |
Other | 4 | 21% |
Unknown | 5 | 26% |