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Mendeley readers
Chapter title |
Production of Protein Kinases in E. coli
|
---|---|
Chapter number | 16 |
Book title |
Heterologous Gene Expression in E.coli
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6887-9_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6885-5, 978-1-4939-6887-9
|
Authors |
Charlotte A. Dodson |
Editors |
Nicola A. Burgess-Brown |
Abstract |
Recombinant protein expression is widely used to generate milligram quantities of protein kinases for crystallographic, enzymatic, or other biophysical assays in vitro. Expression in E. coli is fast, cheap, and reliable. Here I present a detailed protocol for the production of human Aurora-A kinase. I begin with transformation of a suitable plasmid into an expression strain of E. coli, followed by growth and harvesting of bacterial cell cultures. Finally, I describe the purification of Aurora-A to homogeneity using immobilized metal affinity and size exclusion chromatographies. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 33% |
Unspecified | 1 | 17% |
Researcher | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Chemical Engineering | 1 | 17% |
Unspecified | 1 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Unknown | 2 | 33% |