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Chapter title |
Cold-Shock Expression System in E. coli for Protein NMR Studies
|
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Chapter number | 23 |
Book title |
Heterologous Gene Expression in E.coli
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6887-9_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6885-5, 978-1-4939-6887-9
|
Authors |
Toshihiko Sugiki, Toshimichi Fujiwara, Chojiro Kojima Ph.D., Chojiro Kojima |
Editors |
Nicola A. Burgess-Brown |
Abstract |
The cold-shock system using the pCold vector is one of the most effective Escherichia coli heterologous protein expression systems. It allows the improvement of the expression level of the protein of interest in a soluble fraction. In this chapter, we describe practical procedures for the overexpression of heterologous protein of interest by using the pCold vector or the single-protein production system. The latter is one of the most advanced pCold technologies for isotope labeling of the target protein and its NMR studies. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 33% |
Researcher | 2 | 22% |
Student > Ph. D. Student | 1 | 11% |
Unknown | 3 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Agricultural and Biological Sciences | 1 | 11% |
Chemistry | 1 | 11% |
Engineering | 1 | 11% |
Unknown | 4 | 44% |