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Mendeley readers
Chapter title |
A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli
|
---|---|
Chapter number | 10 |
Book title |
Heterologous Gene Expression in E.coli
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6887-9_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6885-5, 978-1-4939-6887-9
|
Authors |
Natalie J. Saez, Ben Cristofori-Armstrong, Raveendra Anangi, Glenn F. King |
Editors |
Nicola A. Burgess-Brown |
Abstract |
Recombinant expression of disulfide-reticulated peptides and proteins is often challenging. We describe a method that exploits the periplasmic disulfide-bond forming machinery of Escherichia coli and combines this with a cleavable, solubility-enhancing fusion tag to obtain higher yields of correctly folded target protein than is achievable via cytoplasmic expression. The protocols provided herein cover all aspects of this approach, from vector construction and transformation to purification of the cleaved target protein and subsequent quality control. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 27% |
Student > Master | 3 | 20% |
Researcher | 2 | 13% |
Student > Ph. D. Student | 1 | 7% |
Professor | 1 | 7% |
Other | 0 | 0% |
Unknown | 4 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 40% |
Agricultural and Biological Sciences | 3 | 20% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Neuroscience | 1 | 7% |
Unknown | 4 | 27% |