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Mendeley readers
Chapter title |
Using Morpholinos to Examine Gene Function During Fin Regeneration
|
---|---|
Chapter number | 7 |
Book title |
Morpholino Oligomers
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6817-6_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6815-2, 978-1-4939-6817-6
|
Authors |
Ryan Thummel, M. Kathryn Iovine Ph.D., M. Kathryn Iovine |
Editors |
Hong M. Moulton, Jon D. Moulton |
Abstract |
In the zebrafish regenerating fin, specific gene-targeting morpholinos have been widely utilized to assess gene function. Unlike in embryos, injection of standard morpholinos in the adult regenerating fin is not sufficient for cellular uptake. Rather, morpholinos are first injected extracellularly into the blastemal compartment, followed by electroporation for cellular uptake. Knockdown phenotypes are evaluated 1-4 days post electroporation. This chapter provides a description of the reagents, equipment, and procedure for successful injection and electroporation of morpholinos into the regenerating fin. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 38% |
Student > Doctoral Student | 1 | 13% |
Other | 1 | 13% |
Student > Master | 1 | 13% |
Professor > Associate Professor | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 50% |
Computer Science | 1 | 13% |
Agricultural and Biological Sciences | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Unknown | 1 | 13% |