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Mendeley readers
| Chapter title |
Delivery of Morpholino Antisense Oligonucleotides to a Developing Ovine Conceptus via Luminal Injection into a Ligated Uterine Horn
|
|---|---|
| Chapter number | 20 |
| Book title |
Morpholino Oligomers
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6817-6_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6815-2, 978-1-4939-6817-6
|
| Authors |
Xiaoqui Wang, Kathrin A. Dunlap |
| Editors |
Hong M. Moulton, Jon D. Moulton |
| Abstract |
In vivo delivery of morpholino antisense oligonucleotides (MAO) directly into the uterine lumen of a peri-implantation period pregnant sheep is an effective technique for evaluation of gene products for conceptus development. The highly phagocytic conceptus is undergoing rapid morphological change, thereby the available MAO are readily consumed and delivered to developing cells. Here, we describe the method for preparation and surgical delivery of MAO-Endo-Porter complex to developing ovine conceptus on day 8 postmating. Also outlined are methods for posttreatment sample recovery on day 16 postmating. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 50% |
| Student > Ph. D. Student | 1 | 25% |
| Researcher | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Veterinary Science and Veterinary Medicine | 1 | 25% |
| Medicine and Dentistry | 1 | 25% |