| Chapter title |
Use of Translation Blocking Morpholinos for Gene Knockdown in Giardia lamblia
|
|---|---|
| Chapter number | 11 |
| Book title |
Morpholino Oligomers
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6817-6_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6815-2, 978-1-4939-6817-6
|
| Authors |
Krtková, Jana, Paredez, Alexander R., Jana Krtková, Alexander R. Paredez |
| Editors |
Hong M. Moulton, Jon D. Moulton |
| Abstract |
Giardia lamblia, a major parasite, is an emerging model organism due to its compact genomic arrangement and composition. The most popular reverse genetic technique, RNAi, is ineffective in Giardia. In contrast, protein depletion by translation blocking morpholinos is suitable for most gene targets and provides up to 80% depletion of the target protein. The method is fast, reliable, and specific. After antisense morpholino oligomer delivery into Giardia trophozoites by electroporation, the cells can be used for many subsequent analyses 8-48 h after treatment. In this chapter, suitable gene tags, plasmids, and techniques necessary for proper morpholino targeting are described. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 18% |
| Student > Ph. D. Student | 2 | 18% |
| Student > Master | 1 | 9% |
| Researcher | 1 | 9% |
| Professor > Associate Professor | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 4 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 45% |
| Social Sciences | 1 | 9% |
| Unknown | 5 | 45% |