Chapter title |
Surface Plasmon Resonance-Based Concentration Determination Assay: Label-Free and Antibody-Free Quantification of Morpholinos
|
---|---|
Chapter number | 21 |
Book title |
Morpholino Oligomers
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6817-6_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6815-2, 978-1-4939-6817-6
|
Authors |
Jordan Boutilier, Hong M. Moulton |
Editors |
Hong M. Moulton, Jon D. Moulton |
Abstract |
Surface plasmon resonance (SPR) is a physical process that allows label-free and real-time detection of biomolecular interactions. SPR provides a rapid and quantitative method for studying interactions of macromolecules such as proteins and nucleic acids. Antisense Morpholino oligomers are widely used to regulate gene expression and the US FDA has approved a Morpholino drug for treatment of Duchenne muscular dystrophy. Here, we describe an antibody-free, label-free, high-throughput, and walk-away SPR method for quantification of Morpholino compounds extracted from biological specimens. This provides a valuable way for determining pharmacokinetics and pharmacodynamics of Morpholino oligomers in biological matrices for research and therapeutic applications. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 20% |
Student > Bachelor | 1 | 10% |
Lecturer > Senior Lecturer | 1 | 10% |
Researcher | 1 | 10% |
Unknown | 5 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Agricultural and Biological Sciences | 1 | 10% |
Neuroscience | 1 | 10% |
Medicine and Dentistry | 1 | 10% |
Unknown | 5 | 50% |