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Mendeley readers
Chapter title |
Ultrasensitive Hybridization-Based ELISA Method for the Determination of Phosphorodiamidate Morpholino Oligonucleotides in Biological samples
|
---|---|
Chapter number | 22 |
Book title |
Morpholino Oligomers
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6817-6_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6815-2, 978-1-4939-6817-6
|
Authors |
Umar Burki, Volker Straub |
Editors |
Hong M. Moulton, Jon D. Moulton |
Abstract |
Determining the concentration of oligonucleotide in biological samples such as tissue lysate and serum is essential for determining the biodistribution and pharmacokinetic profile, respectively. ELISA-based assays have shown far greater sensitivities compared to other methods such as HPLC and LC/MS. Here, we describe a novel ultrasensitive hybridization-based ELISA method for quantitating morpholino oligonucleotides in mouse tissue lysate and serum samples. The assay has a linear detection range of 5-250 pM (R2 > 0.99). |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 13% |
Student > Bachelor | 1 | 13% |
Researcher | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 4 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 2 | 25% |
Agricultural and Biological Sciences | 1 | 13% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Unknown | 4 | 50% |