| Chapter title |
Blocking Zebrafish MicroRNAs with Morpholinos
|
|---|---|
| Chapter number | 6 |
| Book title |
Morpholino Oligomers
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6817-6_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6815-2, 978-1-4939-6817-6
|
| Authors |
Flynt, Alex Sutton, Rao, Mahesh, Patton, James G., Alex Sutton Flynt, Mahesh Rao, James G. Patton |
| Editors |
Hong M. Moulton, Jon D. Moulton |
| Abstract |
Antisense morpholino oligonucleotides have been commonly used in zebrafish to inhibit mRNA function, either by inhibiting pre-mRNA splicing or by blocking translation initiation. Even with the advent of genome editing by CRISP/Cas9 technology, morpholinos provide a useful and rapid tool to knockdown gene expression. This is especially true when dealing with multiple alleles and large gene families where genetic redundancy can complicate knockout of all family members. miRNAs are small noncoding RNAs that are often encoded in gene families and can display extensive genetic redundancy. This redundancy, plus their small size which can limit targeting by CRISPR/Cas9, makes morpholino-based strategies particularly attractive for inhibition of miRNA function. We provide the rationale, background, and methods to inhibit miRNA function with antisense morpholinos during early development and in the adult retina in zebrafish. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 25% |
| Researcher | 5 | 21% |
| Student > Master | 3 | 13% |
| Student > Bachelor | 2 | 8% |
| Professor | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 4 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 42% |
| Immunology and Microbiology | 3 | 13% |
| Medicine and Dentistry | 2 | 8% |
| Agricultural and Biological Sciences | 1 | 4% |
| Unspecified | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 25% |