| Chapter title |
Effects of Macrophage Conditioned-Medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion
|
|---|---|
| Chapter number | 17 |
| Book title |
Muscle Stem Cells
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6771-1_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6769-8, 978-1-4939-6771-1
|
| Authors |
Marielle Saclier, Marine Theret, Rémi Mounier, Bénédicte Chazaud |
| Editors |
Eusebio Perdiguero, DDW Cornelison |
| Abstract |
Skeletal muscle is a highly plastic tissue, which is able to regenerate after an injury. Effective and complete regeneration requires interactions between myogenic precursor cells and several cell types such as macrophages . Bone marrow derived macrophages in mouse and monocyte-derived macrophages in human are useful tools to obtain macrophage populations that may be specifically activated/polarized in vitro (e.g., pro-inflammatory, anti-inflammatory, and alternatively activated macrophages ). In vitro , human or murine primary myogenic cells recapitulate the adult myogenesis program through proliferation, myogenic differentiation, and fusion. Macrophages being highly secreting cells, they act on various biological processes including adult myogenesis . Here, we present protocols to analyze in vitro the effect of macrophage-secreted factors on muscle cell proliferation or differentiation in both mouse and human. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 7% |
| Unknown | 14 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 27% |
| Student > Ph. D. Student | 3 | 20% |
| Student > Bachelor | 2 | 13% |
| Unspecified | 1 | 7% |
| Student > Master | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 27% |
| Biochemistry, Genetics and Molecular Biology | 3 | 20% |
| Medicine and Dentistry | 2 | 13% |
| Unspecified | 1 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 20% |