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Muscle Stem Cells

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Cover of 'Muscle Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Muscle Stem Cells: A Model System for Adult Stem Cell Biology
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    Chapter 2 Isolation of Muscle Stem Cells from Mouse Skeletal Muscle
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    Chapter 3 Primary Mouse Myoblast Purification using Magnetic Cell Separation
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    Chapter 4 Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell
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    Chapter 5 Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors
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    Chapter 6 Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells
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    Chapter 7 Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle
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    Chapter 8 Isolation and Characterization of Vessel-Associated Stem/Progenitor Cells from Skeletal Muscle
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    Chapter 9 Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture
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    Chapter 10 Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells
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    Chapter 11 Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle
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    Chapter 12 Transplantation of Skeletal Muscle Stem Cells
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    Chapter 13 Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells
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    Chapter 14 Monitoring Autophagy in Muscle Stem Cells
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    Chapter 15 Mimicking Muscle Stem Cell Quiescence in Culture: Methods for Synchronization in Reversible Arrest
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    Chapter 16 Methods for Observing and Quantifying Muscle Satellite Cell Motility and Invasion In Vitro
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    Chapter 17 Effects of Macrophage Conditioned-Medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion
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    Chapter 18 Optimization of Satellite Cell Culture Through Biomaterials
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    Chapter 19 Systematic Identification of Genes Regulating Muscle Stem Cell Self-Renewal and Differentiation
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    Chapter 20 Bioinformatics for Novel Long Intergenic Noncoding RNA (lincRNA) Identification in Skeletal Muscle Cells
Attention for Chapter 2: Isolation of Muscle Stem Cells from Mouse Skeletal Muscle
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Chapter title
Isolation of Muscle Stem Cells from Mouse Skeletal Muscle
Chapter number 2
Book title
Muscle Stem Cells
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6771-1_2
Pubmed ID
Book ISBNs
978-1-4939-6769-8, 978-1-4939-6771-1
Authors

Barbara Gayraud-Morel, Francesca Pala, Hiroshi Sakai, Shahragim Tajbakhsh

Editors

Eusebio Perdiguero, DDW Cornelison

Abstract

Isolation of muscle stem cells from skeletal muscle is a critical step for the study of skeletal myogenesis and regeneration. Although stem cell isolation has been performed for decades, the emergence of flow cytometry with defined cell surface markers, or transgenic mouse models, has allowed the efficient isolation of highly enriched stem cell populations. Here, we describe the isolation of mouse muscle stem cells using two different combinations of enzyme treatments allowing the release of mononucleated muscle stem cells from their niche. Mouse muscle stem cells can be further isolated as a highly enriched population by flow cytometry using fluorescent reporters or cell surface markers. We will present advantages and drawbacks of these different approaches.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 23%
Researcher 2 15%
Other 1 8%
Student > Doctoral Student 1 8%
Student > Bachelor 1 8%
Other 3 23%
Unknown 2 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 38%
Agricultural and Biological Sciences 2 15%
Chemical Engineering 1 8%
Sports and Recreations 1 8%
Neuroscience 1 8%
Other 1 8%
Unknown 2 15%