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Muscle Stem Cells

Overview of attention for book
Cover of 'Muscle Stem Cells'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Muscle Stem Cells: A Model System for Adult Stem Cell Biology
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    Chapter 2 Isolation of Muscle Stem Cells from Mouse Skeletal Muscle
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    Chapter 3 Primary Mouse Myoblast Purification using Magnetic Cell Separation
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    Chapter 4 Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell
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    Chapter 5 Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors
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    Chapter 6 Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells
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    Chapter 7 Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle
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    Chapter 8 Isolation and Characterization of Vessel-Associated Stem/Progenitor Cells from Skeletal Muscle
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    Chapter 9 Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture
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    Chapter 10 Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells
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    Chapter 11 Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle
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    Chapter 12 Transplantation of Skeletal Muscle Stem Cells
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    Chapter 13 Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells
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    Chapter 14 Monitoring Autophagy in Muscle Stem Cells
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    Chapter 15 Mimicking Muscle Stem Cell Quiescence in Culture: Methods for Synchronization in Reversible Arrest
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    Chapter 16 Methods for Observing and Quantifying Muscle Satellite Cell Motility and Invasion In Vitro
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    Chapter 17 Effects of Macrophage Conditioned-Medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion
  19. Altmetric Badge
    Chapter 18 Optimization of Satellite Cell Culture Through Biomaterials
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    Chapter 19 Systematic Identification of Genes Regulating Muscle Stem Cell Self-Renewal and Differentiation
  21. Altmetric Badge
    Chapter 20 Bioinformatics for Novel Long Intergenic Noncoding RNA (lincRNA) Identification in Skeletal Muscle Cells
Attention for Chapter 9: Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture
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Chapter title
Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture
Chapter number 9
Book title
Muscle Stem Cells
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6771-1_9
Pubmed ID
28247350
Book ISBNs
978-1-4939-6769-8, 978-1-4939-6771-1
Authors

Marcela Low, Christine Eisner, Fabio Rossi, Low, Marcela, Eisner, Christine, Rossi, Fabio

Editors

Eusebio Perdiguero, DDW Cornelison

Abstract

Fibro/adipogenic progenitors (FAPs ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP populations from various tissues. Here, we describe a protocol for the isolation and cell culture of FAPs from murine skeletal muscle using fluorescence -activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell culture of FAPs represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases.

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Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 44 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 23%
Student > Ph. D. Student 6 14%
Student > Master 5 11%
Researcher 3 7%
Student > Doctoral Student 1 2%
Other 5 11%
Unknown 14 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 30%
Agricultural and Biological Sciences 6 14%
Medicine and Dentistry 5 11%
Engineering 4 9%
Unknown 16 36%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 April 2017.
All research outputs
#20,408,464
of 22,958,253 outputs
Outputs from Methods in molecular biology
#9,919
of 13,137 outputs
Outputs of similar age
#271,148
of 311,244 outputs
Outputs of similar age from Methods in molecular biology
#212
of 268 outputs
Altmetric has tracked 22,958,253 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,137 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 311,244 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 268 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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