| Chapter title |
Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture
|
|---|---|
| Chapter number | 9 |
| Book title |
Muscle Stem Cells
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6771-1_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6769-8, 978-1-4939-6771-1
|
| Authors |
Marcela Low, Christine Eisner, Fabio Rossi, Low, Marcela, Eisner, Christine, Rossi, Fabio |
| Editors |
Eusebio Perdiguero, DDW Cornelison |
| Abstract |
Fibro/adipogenic progenitors (FAPs ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP populations from various tissues. Here, we describe a protocol for the isolation and cell culture of FAPs from murine skeletal muscle using fluorescence -activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell culture of FAPs represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 49 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 20% |
| Student > Master | 7 | 14% |
| Student > Ph. D. Student | 6 | 12% |
| Researcher | 4 | 8% |
| Student > Doctoral Student | 1 | 2% |
| Other | 6 | 12% |
| Unknown | 15 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 14 | 29% |
| Agricultural and Biological Sciences | 6 | 12% |
| Medicine and Dentistry | 5 | 10% |
| Engineering | 4 | 8% |
| Unspecified | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 18 | 37% |