Chapter title |
Engineering of Conditional Class I Hdac Knockout Mice and Generation of a Time-Spatial Knockout by a Dual Recombination System
|
---|---|
Chapter number | 14 |
Book title |
HDAC/HAT Function Assessment and Inhibitor Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6527-4_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6525-0, 978-1-4939-6527-4
|
Authors |
Sieglinde Bayer, Matthias Wirth |
Abstract |
The protein sequences of class I HDACs in mice and humans are 96-99 % identical. These highly conserved proteins have crucial roles in biological processes, such as proliferation and development, which is reflected in the lethality that occurs in conventional whole body knockout mice. Therefore, conditional knockouts are inevitable to investigate the functions of class I HDACs in mice. Here, we describe the generation of conditional class I Hdac knockout mice, using Hdac1 as an example. We explain a relatively quick procedure to generate the necessary target vectors by recombination-mediated genetic engineering and gateway techniques. Furthermore, we show how to culture, target, and screen for positively recombined ES cells. Additionally, we present a dual recombination system, which allows the deletion of class I Hdacs at any time by a tamoxifen inducible Cre. |
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Geographical breakdown
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Unknown | 6 | 100% |
Demographic breakdown
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Student > Master | 2 | 33% |
Student > Bachelor | 1 | 17% |
Unspecified | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
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Unspecified | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Unknown | 2 | 33% |