Chapter title |
Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases
|
---|---|
Chapter number | 20 |
Book title |
HDAC/HAT Function Assessment and Inhibitor Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6527-4_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6525-0, 978-1-4939-6527-4
|
Authors |
Benjamin Y. Owusu, Lidija Klampfer |
Abstract |
The luciferase (LUC) reporter assay is commonly used to study gene expression at the transcriptional level. It is convenient, fast, sensitive, inexpensive, and provides quantitative data about small changes in transcription. Signal transducer and activator of transcription 1 (STAT1) is a transcription factor that plays a crucial role in signaling by interferons (IFNs). Here, we describe LUC reporter studies that address the role of histone deacetylase (HDAC) activity in STAT1-dependent gene activation. These experiments include overexpression of HDAC1, HDAC2, HDAC3, and HDAC4 as well as silencing of HDAC1, HDAC2, and HDAC3 through RNA interference in mammalian cancer cells. |
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Unknown | 8 | 100% |
Demographic breakdown
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Student > Bachelor | 2 | 25% |
Student > Doctoral Student | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
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Unknown | 2 | 25% |