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Mendeley readers
Chapter title |
Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization
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Chapter number | 4 |
Book title |
HDAC/HAT Function Assessment and Inhibitor Development
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6527-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6525-0, 978-1-4939-6527-4
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Authors |
Britta Unruhe-Knauf, Shirley K. Knauer |
Abstract |
The chromosomal passenger complex (CPC) is a key regulator of cell division. Its proper localization during the different phases of mitosis and cytokinesis is crucial for the exertion of its various functions. HDACi treatment has been demonstrated to disturb the centromeric localization of the CPC in tumor cells, thus leading to severe mitotic defects often followed by apoptosis. In this chapter, we describe how HDACi-induced changes of the CPC localization can be analyzed by indirect immunofluorescence using CPC-specific primary and fluorophore-coupled secondary antibodies followed by confocal microscopy. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 50% |
Student > Ph. D. Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Environmental Science | 1 | 25% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Medicine and Dentistry | 1 | 25% |
Unknown | 1 | 25% |