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Mendeley readers
Chapter title |
Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques
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Chapter number | 25 |
Book title |
HDAC/HAT Function Assessment and Inhibitor Development
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6527-4_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6525-0, 978-1-4939-6527-4
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Authors |
Tobias Wagner, Maren Godmann, Thorsten Heinzel |
Abstract |
Histone deacetylases (HDACs) are controlling dynamic protein acetylation by removing acetyl moieties from lysine. Histone deacetylases themselves are regulated on the posttranslational level, including modifications with small ubiquitin-like modifier (SUMO) proteins. Detecting SUMO modifications of deacetylases by immunoblotting is technically challenging due to the typically low ratio of the modified compared to the unmodified species. Here, we describe a set of methods for the detection of endogenous sumoylated HDACs by immunoprecipitation and immunoblotting techniques. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 50% |
Lecturer > Senior Lecturer | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 100% |