Chapter title |
How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot
|
---|---|
Chapter number | 26 |
Book title |
HDAC/HAT Function Assessment and Inhibitor Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6527-4_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6525-0, 978-1-4939-6527-4
|
Authors |
Mandy Beyer, Nicole Kiweler, Siavosh Mahboobi, Oliver H. Krämer |
Abstract |
Histone deacetylases (HDACs) catalyze the deacetylation of lysine residues in their target proteins. This biochemical modification can have profound effects on the functions of these proteins and a dysregulation of HDAC activity contributes to severe diseases, including neoplastic transformation. In the following chapter, we present a strategy that allows to distinguish between the inhibition of the class I HDACs HDAC1, 2, and 3 and of the class IIb HDAC HDAC6. This method is based on Western blot and relies on the detection of hyperacetylated substrates of class I or class IIb HDACs in lysates from cells that were treated with histone deacetylase inhibitors (HDACi). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Unspecified | 3 | 38% |
Professor | 1 | 13% |
Researcher | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
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Unspecified | 3 | 38% |
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Chemistry | 1 | 13% |
Unknown | 3 | 38% |