| Chapter title |
Immunological Identification of Fungal Species.
|
|---|---|
| Chapter number | 20 |
| Book title |
Human Fungal Pathogen Identification
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6515-1_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6513-7, 978-1-4939-6515-1
|
| Authors |
Filomena Nogueira, Fabian Istel, Leonel Pereira, Michael Tscherner, Karl Kuchler |
| Editors |
Thomas Lion |
| Abstract |
Immunodetection is described in this chapter as a technique for producing specific antibodies for antigen detection of the major human fungal pathogens. In the case of Candida spp., heat-killed cells are used to immunize mice over a couple of weeks and then splenocytes are isolated and further fused with myelomas to easily propagate the antibodies produced in the mice. The resulting antibodies follow a purification process where antibody levels and concentrations are determined. Fungal cells are also lysed to obtain whole cell extracts as a prior step for identification of antigens using immunoprecipitation. Finally, this method permits the production of specific antibodies against fungi and the identification of the respective antigens in an in vivo model. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Brazil | 1 | 6% |
| Unknown | 15 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 3 | 19% |
| Student > Postgraduate | 3 | 19% |
| Student > Master | 2 | 13% |
| Researcher | 2 | 13% |
| Student > Ph. D. Student | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 25% |
| Biochemistry, Genetics and Molecular Biology | 3 | 19% |
| Unspecified | 1 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 31% |