| Chapter title |
Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cells in the Infected Tissue.
|
|---|---|
| Chapter number | 8 |
| Book title |
Human Fungal Pathogen Identification
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6515-1_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6513-7, 978-1-4939-6515-1
|
| Authors |
Florian Sparber, Salomé LeibundGut-Landmann |
| Editors |
Thomas Lion |
| Abstract |
The immune system is important to protect the host from fungal infections. Diverse cell types belonging to the innate or adaptive branch of the immune system act in a tightly coordinated and tissue-specific manner. Experimental mouse models of fungal infections have proved essential for assessing the protective principles against different fungal pathogens. Besides pathological, histological, biochemical and molecular parameters, the analysis of phenotypic and functional aspects of immune cells in infected tissues is key for understanding the mechanisms of antifungal defense. In this chapter, we describe a method based on flow cytometry to assess innate and adaptive immune cells isolated from an in vivo context in a qualitative and quantitative manner. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 10% |
| Unknown | 9 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 20% |
| Student > Ph. D. Student | 1 | 10% |
| Student > Postgraduate | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Professor > Associate Professor | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 4 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 20% |
| Biochemistry, Genetics and Molecular Biology | 1 | 10% |
| Immunology and Microbiology | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Unknown | 5 | 50% |