Chapter title |
Human Fungal Pathogen Identification
|
---|---|
Chapter number | 21 |
Book title |
Human Fungal Pathogen Identification
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6515-1_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6513-7, 978-1-4939-6515-1
|
Authors |
Grumaz, Christian, Kirstahler, Philipp, Sohn, Kai, Christian Grumaz, Philipp Kirstahler, Kai Sohn |
Editors |
Thomas Lion |
Abstract |
Sequencing the whole genome of an organism is invaluable for its comprehensive molecular characterization and has been drastically facilitated by the advent of high-throughput sequencing techniques. Especially in clinical microbiology the impact of sequenced strains increases as resistance and virulence markers can easily be detected. Here, we describe a combined approach for sequencing a fungal genome and transcriptome from initial nucleic acid isolation through the generation of ready-to-load DNA libraries for the Illumina platform and the final step of genome assembly with subsequent gene annotation. |
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