Chapter title |
Fungal-Grade Reagents and Materials for Molecular Analysis.
|
---|---|
Chapter number | 6 |
Book title |
Human Fungal Pathogen Identification
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6515-1_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6513-7, 978-1-4939-6515-1
|
Authors |
Michael G. Lorenz, Michael Lustig, Marina Linow |
Editors |
Thomas Lion |
Abstract |
Fungal DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection. |
Mendeley readers
Geographical breakdown
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 1 | 33% |
Researcher | 1 | 33% |
Student > Master | 1 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Chemistry | 1 | 33% |
Medicine and Dentistry | 1 | 33% |