| Chapter title |
Genus- and Species-Specific PCR Detection Methods.
|
|---|---|
| Chapter number | 15 |
| Book title |
Human Fungal Pathogen Identification
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6515-1_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6513-7, 978-1-4939-6515-1
|
| Authors |
Jan Springer, Jürgen Löffler |
| Editors |
Thomas Lion |
| Abstract |
PCR-based detection of fungal pathogens offers a sensitive and specific tool for the diagnosis of invasive fungal infections. A large variety of different clinical specimen types can be used as original material. However, certain precautions, in addition to the published MIQE guidelines [1], need to be taken to prevent contaminations from airborne fungal spores and PCR reagents. In addition, the European Aspergillus PCR Initiative (EAPCRI) recently defined standards for Aspergillus PCR [2, 3], following these recommendations leads to superior sensitivity. The combination of fungal PCR with the galactomannan ELISA assay increases the sensitivity for the detection of Aspergillus DNA from blood, compared to a single assay only [4, 5]. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 22% |
| Lecturer | 1 | 11% |
| Other | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Unknown | 4 | 44% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 33% |
| Agricultural and Biological Sciences | 1 | 11% |
| Computer Science | 1 | 11% |
| Unknown | 4 | 44% |