| Chapter title |
Bioinformatics Analysis of Small RNA Transcriptomes: The Detailed Workflow.
|
|---|---|
| Chapter number | 16 |
| Book title |
Plant Epigenetics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4899-7708-3_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4899-7706-9, 978-1-4899-7708-3
|
| Authors |
Slava Ilnytskyy, Andriy Bilichak |
| Editors |
Igor Kovalchuk |
| Abstract |
Next-generation sequencing became a method of choice for the investigation of small RNA transcriptomes in plants and animals. Although a technical side of sequencing itself is becoming routine, and experimental costs are affordable, data analysis still remains a challenge, especially for researchers with limited computational experience. Here, we present a detailed description of a computational workflow designed to take raw sequencing reads as input, to obtain small RNA predictions, and to detect the differentially expressed microRNAs as a result. The exact commands and pieces of code are provided and hopefully can be adapted and used by other researchers to facilitate the study of small RNA regulation. |
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Mendeley readers
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| Student > Bachelor | 2 | 14% |
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