| Chapter title |
Differentially Methylated Region-Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify DMRs in Uncharacterized Genomes.
|
|---|---|
| Chapter number | 10 |
| Book title |
Plant Epigenetics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4899-7708-3_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4899-7706-9, 978-1-4899-7708-3
|
| Authors |
Pavlina Sasheva, Ueli Grossniklaus |
| Editors |
Igor Kovalchuk |
| Abstract |
Over the last years, it has become increasingly clear that environmental influences can affect the epigenomic landscape and that some epigenetic variants can have heritable, phenotypic effects. While there are a variety of methods to perform genome-wide analyses of DNA methylation in model organisms, this is still a challenging task for non-model organisms without a reference genome. Differentially methylated region-representational difference analysis (DMR-RDA) is a sensitive and powerful PCR-based technique that isolates DNA fragments that are differentially methylated between two otherwise identical genomes. The technique does not require special equipment and is independent of prior knowledge about the genome. It is even applicable to genomes that have high complexity and a large size, being the method of choice for the analysis of plant non-model systems. |
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Mendeley readers
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| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 25% |
| Student > Ph. D. Student | 1 | 25% |
| Researcher | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
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| Agricultural and Biological Sciences | 2 | 50% |
| Nursing and Health Professions | 1 | 25% |
| Unknown | 1 | 25% |