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Plant Epigenetics

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Cover of 'Plant Epigenetics'

Table of Contents

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    Book Overview
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    Chapter 1 Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis.
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    Chapter 2 Chromatin Conformation Capture-Based Analysis of Nuclear Architecture.
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    Chapter 3 Meta-analysis of Genome-Wide Chromatin Data.
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    Chapter 4 Localization of miRNAs by In Situ Hybridization in Plants Using Conventional Oligonucleotide Probes.
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    Chapter 5 The Combined Bisulfite Restriction Analysis (COBRA) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns.
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    Chapter 6 Analysis of Global Genome Methylation Using the Cytosine-Extension Assay.
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    Chapter 7 In Situ Analysis of DNA Methylation in Plants.
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    Chapter 8 Plant Epigenetics
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    Chapter 9 Analysis of DNA Cytosine Methylation Patterns Using Methylation-Sensitive Amplification Polymorphism (MSAP).
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    Chapter 10 Differentially Methylated Region-Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify DMRs in Uncharacterized Genomes.
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    Chapter 11 Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays.
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    Chapter 12 Northern Blotting Techniques for Small RNAs.
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    Chapter 13 Stem-Loop qRT-PCR for the Detection of Plant microRNAs.
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    Chapter 14 Profiling New Small RNA Sequences.
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    Chapter 15 Small RNA Library Preparation and Illumina Sequencing in Plants.
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    Chapter 16 Bioinformatics Analysis of Small RNA Transcriptomes: The Detailed Workflow.
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    Chapter 17 Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression Using Virus-Induced Gene Silencing.
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    Chapter 18 The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks.
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    Chapter 19 Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species.
Attention for Chapter 19: Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species.
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Chapter title
Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species.
Chapter number 19
Book title
Plant Epigenetics
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4899-7708-3_19
Pubmed ID
Book ISBNs
978-1-4899-7706-9, 978-1-4899-7708-3
Authors

Christian Parisod

Editors

Igor Kovalchuk

Abstract

Taking transposable elements into consideration in surveys of genetic and epigenetic variation remains challenging in species lacking a high-quality reference genome. Here, molecular techniques reducing genome complexity and specifically targeting restructuring and methylation changes in TE genome fractions are described. In particular, methyl-sensitive transposon display (MSTD) uses isoschizomers and PCR amplifications to assess the methylation environment of TE insertions. MSTD offers reliable insights into genome-wide epigenetic changes associated with TEs, especially when used together with similar techniques tracking random sequences.

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 50%
Student > Bachelor 1 25%
Student > Ph. D. Student 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 100%