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Plant Epigenetics

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Cover of 'Plant Epigenetics'

Table of Contents

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    Book Overview
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    Chapter 1 Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis.
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    Chapter 2 Chromatin Conformation Capture-Based Analysis of Nuclear Architecture.
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    Chapter 3 Meta-analysis of Genome-Wide Chromatin Data.
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    Chapter 4 Localization of miRNAs by In Situ Hybridization in Plants Using Conventional Oligonucleotide Probes.
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    Chapter 5 The Combined Bisulfite Restriction Analysis (COBRA) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns.
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    Chapter 6 Analysis of Global Genome Methylation Using the Cytosine-Extension Assay.
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    Chapter 7 In Situ Analysis of DNA Methylation in Plants.
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    Chapter 8 Plant Epigenetics
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    Chapter 9 Analysis of DNA Cytosine Methylation Patterns Using Methylation-Sensitive Amplification Polymorphism (MSAP).
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    Chapter 10 Differentially Methylated Region-Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify DMRs in Uncharacterized Genomes.
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    Chapter 11 Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays.
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    Chapter 12 Northern Blotting Techniques for Small RNAs.
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    Chapter 13 Stem-Loop qRT-PCR for the Detection of Plant microRNAs.
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    Chapter 14 Profiling New Small RNA Sequences.
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    Chapter 15 Small RNA Library Preparation and Illumina Sequencing in Plants.
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    Chapter 16 Bioinformatics Analysis of Small RNA Transcriptomes: The Detailed Workflow.
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    Chapter 17 Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression Using Virus-Induced Gene Silencing.
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    Chapter 18 The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks.
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    Chapter 19 Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species.
Attention for Chapter 7: In Situ Analysis of DNA Methylation in Plants.
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Chapter title
In Situ Analysis of DNA Methylation in Plants.
Chapter number 7
Book title
Plant Epigenetics
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4899-7708-3_7
Pubmed ID
Book ISBNs
978-1-4899-7706-9, 978-1-4899-7708-3
Authors

Palak Kathiria, Igor Kovalchuk

Editors

Igor Kovalchuk

Abstract

Epigenetic regulation in the plant genome is associated with the determination of expression patterns of various genes. Methylation of DNA at cytosine residues is one of the mechanisms of epigenetic regulation and has been a subject of various studies. Various techniques have been developed to analyze DNA methylation, most of which involve isolation of chromatin from cells and further in vitro studies. Limited techniques are available for in situ study of DNA methylation in plants. Here, we present such an in situ method for DNA methylation analysis which has high sensitivity and good reproducibility.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 22%
Researcher 2 22%
Student > Bachelor 1 11%
Student > Master 1 11%
Student > Doctoral Student 1 11%
Other 0 0%
Unknown 2 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 44%
Biochemistry, Genetics and Molecular Biology 2 22%
Nursing and Health Professions 1 11%
Unknown 2 22%