| Chapter title |
Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression Using Virus-Induced Gene Silencing.
|
|---|---|
| Chapter number | 17 |
| Book title |
Plant Epigenetics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4899-7708-3_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4899-7706-9, 978-1-4899-7708-3
|
| Authors |
Andriy Bilichak, Igor Kovalchuk |
| Editors |
Igor Kovalchuk |
| Abstract |
Virus-induced gene silencing (VIGS) is a powerful epigenetic tool that allows in a relatively short period of time to down-regulate the expression of an endogenous gene in infected plants for either monitoring the resulting phenotype or enhancing/modifying a particular trait associated with the gene. Here, we describe the utilization of Tobacco rattle virus (TRV) as a vector for the VIGS technique in Arabidopsis plants. The unique ability of TRV to infect both somatic tissues and gametes allows deciphering the role of genes in these tissues simultaneously. As an example, we demonstrate the utilization of TRV to down-regulate the expression of AGO2 and NRPD1a genes in ovules of Arabidopsis plants in order to boost the stable transformation efficiency by floral dip. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Student > Master | 2 | 20% |
| Student > Bachelor | 1 | 10% |
| Student > Doctoral Student | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Agricultural and Biological Sciences | 4 | 40% |
| Unknown | 2 | 20% |