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Protein Chromatography

Overview of attention for book
Cover of 'Protein Chromatography'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 A Synopsis of Proteins and Their Purification.
  3. Altmetric Badge
    Chapter 2 Gel-Filtration Chromatography.
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    Chapter 3 Immunoaffinity Chromatography: Concepts and Applications.
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    Chapter 4 Avoiding Proteolysis During Protein Purification.
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    Chapter 5 Scale-Up of Protein Purification: Downstream Processing Issues.
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    Chapter 6 Phage Display: A Powerful Technology for the Generation of High-Specificity Affinity Reagents from Alternative Immune Sources.
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    Chapter 7 Protein Stability: Enhancement and Measurement.
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    Chapter 8 Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.
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    Chapter 9 Storage and Lyophilization of Pure Proteins.
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    Chapter 10 Differential Precipitation and Solubilization of Proteins.
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    Chapter 11 Ion-Exchange Chromatography: Basic Principles and Application.
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    Chapter 12 Protein Quantitation and Analysis of Purity.
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    Chapter 13 Purification of Proteins Fused to Maltose-Binding Protein.
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    Chapter 14 Purification of Polyhistidine-Tagged Proteins.
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    Chapter 15 Purification of Antibodies Using Affinity Chromatography.
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    Chapter 16 Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens.
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    Chapter 17 Measuring Protein-Protein Interactions Using Biacore.
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    Chapter 18 Hydrophobic Interaction Chromatography.
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    Chapter 19 Fast Protein Liquid Chromatography.
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    Chapter 20 Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems.
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    Chapter 21 Strategies for the Purification of Membrane Proteins.
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    Chapter 22 Antimicrobial Peptide Production and Purification.
  24. Altmetric Badge
    Chapter 23 Lectin Affinity Chromatography (LAC).
Attention for Chapter 2: Gel-Filtration Chromatography.
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Good Attention Score compared to outputs of the same age and source (72nd percentile)

Mentioned by

patent
1 patent

Citations

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23 Dimensions

Readers on

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617 Mendeley
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Chapter title
Gel-Filtration Chromatography.
Chapter number 2
Book title
Protein Chromatography
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6412-3_2
Pubmed ID
Book ISBNs
978-1-4939-6410-9, 978-1-4939-6412-3
Authors

Ciarán Ó’Fágáin, Philip M. Cummins, Brendan F. O’Connor, Ó’Fágáin, Ciarán, Cummins, Philip M., O’Connor, Brendan F.

Editors

Dermot Walls, Sinéad T. Loughran

Abstract

Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 617 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Hungary 1 <1%
Germany 1 <1%
Unknown 615 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 151 24%
Student > Ph. D. Student 71 12%
Student > Master 49 8%
Researcher 45 7%
Student > Doctoral Student 21 3%
Other 41 7%
Unknown 239 39%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 156 25%
Agricultural and Biological Sciences 69 11%
Chemistry 46 7%
Engineering 21 3%
Pharmacology, Toxicology and Pharmaceutical Science 17 3%
Other 59 10%
Unknown 249 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2019.
All research outputs
#7,874,017
of 23,870,803 outputs
Outputs from Methods in molecular biology
#2,450
of 13,453 outputs
Outputs of similar age
#143,565
of 425,230 outputs
Outputs of similar age from Methods in molecular biology
#246
of 1,071 outputs
Altmetric has tracked 23,870,803 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,453 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 425,230 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 1,071 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.