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Protein Chromatography

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Cover of 'Protein Chromatography'

Table of Contents

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    Book Overview
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    Chapter 1 A Synopsis of Proteins and Their Purification.
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    Chapter 2 Gel-Filtration Chromatography.
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    Chapter 3 Immunoaffinity Chromatography: Concepts and Applications.
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    Chapter 4 Avoiding Proteolysis During Protein Purification.
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    Chapter 5 Scale-Up of Protein Purification: Downstream Processing Issues.
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    Chapter 6 Phage Display: A Powerful Technology for the Generation of High-Specificity Affinity Reagents from Alternative Immune Sources.
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    Chapter 7 Protein Stability: Enhancement and Measurement.
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    Chapter 8 Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.
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    Chapter 9 Storage and Lyophilization of Pure Proteins.
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    Chapter 10 Differential Precipitation and Solubilization of Proteins.
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    Chapter 11 Ion-Exchange Chromatography: Basic Principles and Application.
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    Chapter 12 Protein Quantitation and Analysis of Purity.
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    Chapter 13 Purification of Proteins Fused to Maltose-Binding Protein.
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    Chapter 14 Purification of Polyhistidine-Tagged Proteins.
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    Chapter 15 Purification of Antibodies Using Affinity Chromatography.
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    Chapter 16 Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens.
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    Chapter 17 Measuring Protein-Protein Interactions Using Biacore.
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    Chapter 18 Hydrophobic Interaction Chromatography.
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    Chapter 19 Fast Protein Liquid Chromatography.
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    Chapter 20 Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems.
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    Chapter 21 Strategies for the Purification of Membrane Proteins.
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    Chapter 22 Antimicrobial Peptide Production and Purification.
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    Chapter 23 Lectin Affinity Chromatography (LAC).
Attention for Chapter 12: Protein Quantitation and Analysis of Purity.
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Chapter title
Protein Quantitation and Analysis of Purity.
Chapter number 12
Book title
Protein Chromatography
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6412-3_12
Pubmed ID
Book ISBNs
978-1-4939-6410-9, 978-1-4939-6412-3
Authors

Eva M. Campion, Sinéad T. Loughran, Dermot Walls

Editors

Dermot Walls, Sinéad T. Loughran

Abstract

The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and is a critical factor in many clinical applications. The large number and variety of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on such factors as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-Polymerase Chain Reaction.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 37 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 24%
Student > Ph. D. Student 5 14%
Student > Master 3 8%
Lecturer 2 5%
Other 2 5%
Other 5 14%
Unknown 11 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 22%
Chemistry 5 14%
Agricultural and Biological Sciences 5 14%
Engineering 2 5%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 4 11%
Unknown 12 32%