Chapter title |
Protein Quantitation and Analysis of Purity.
|
---|---|
Chapter number | 12 |
Book title |
Protein Chromatography
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6412-3_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6410-9, 978-1-4939-6412-3
|
Authors |
Eva M. Campion, Sinéad T. Loughran, Dermot Walls |
Editors |
Dermot Walls, Sinéad T. Loughran |
Abstract |
The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and is a critical factor in many clinical applications. The large number and variety of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on such factors as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-Polymerase Chain Reaction. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 9 | 24% |
Student > Ph. D. Student | 5 | 14% |
Student > Master | 3 | 8% |
Lecturer | 2 | 5% |
Other | 2 | 5% |
Other | 5 | 14% |
Unknown | 11 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 22% |
Chemistry | 5 | 14% |
Agricultural and Biological Sciences | 5 | 14% |
Engineering | 2 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 4 | 11% |
Unknown | 12 | 32% |