| Chapter title |
Phage Display: A Powerful Technology for the Generation of High-Specificity Affinity Reagents from Alternative Immune Sources.
|
|---|---|
| Chapter number | 6 |
| Book title |
Protein Chromatography
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6412-3_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6410-9, 978-1-4939-6412-3
|
| Authors |
William J. J. Finlay, Laird Bloom, Joanne Grant, Edward Franklin, Deirdre Ní Shúilleabháin, Orla Cunningham, Finlay, William J. J., Bloom, Laird, Grant, Joanne, Franklin, Edward, Shúilleabháin, Deirdre Ní, Cunningham, Orla |
| Editors |
Dermot Walls, Sinéad T. Loughran |
| Abstract |
Antibodies are critical reagents in many fundamental biochemical methods such as affinity chromatography, enzyme-linked immunosorbent assays (ELISA), flow cytometry, western blotting, immunoprecipitation, and immunohistochemistry techniques. As our understanding of the proteome becomes more complex, demand is rising for rapidly generated antibodies of higher specificity than ever before. It is therefore surprising that few investigators have moved beyond the classical methods of antibody production in their search for new reagents. Despite their long-standing efficacy, recombinant antibody generation technologies such as phage display are still largely the tools of biotechnology companies or research groups with a direct interest in protein engineering. In this chapter, we discuss the inherent limitations of classical polyclonal and monoclonal antibody generation and highlight an attractive alternative: generating high-specificity, high-affinity recombinant antibodies from alternative immune sources such as chickens, via phage display. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 21% |
| Student > Ph. D. Student | 7 | 18% |
| Student > Master | 5 | 13% |
| Student > Bachelor | 4 | 11% |
| Other | 3 | 8% |
| Other | 5 | 13% |
| Unknown | 6 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 32% |
| Agricultural and Biological Sciences | 8 | 21% |
| Immunology and Microbiology | 3 | 8% |
| Chemistry | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Other | 4 | 11% |
| Unknown | 7 | 18% |