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Protein Chromatography

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Cover of 'Protein Chromatography'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 A Synopsis of Proteins and Their Purification.
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    Chapter 2 Gel-Filtration Chromatography.
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    Chapter 3 Immunoaffinity Chromatography: Concepts and Applications.
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    Chapter 4 Avoiding Proteolysis During Protein Purification.
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    Chapter 5 Scale-Up of Protein Purification: Downstream Processing Issues.
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    Chapter 6 Phage Display: A Powerful Technology for the Generation of High-Specificity Affinity Reagents from Alternative Immune Sources.
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    Chapter 7 Protein Stability: Enhancement and Measurement.
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    Chapter 8 Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.
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    Chapter 9 Storage and Lyophilization of Pure Proteins.
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    Chapter 10 Differential Precipitation and Solubilization of Proteins.
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    Chapter 11 Ion-Exchange Chromatography: Basic Principles and Application.
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    Chapter 12 Protein Quantitation and Analysis of Purity.
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    Chapter 13 Purification of Proteins Fused to Maltose-Binding Protein.
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    Chapter 14 Purification of Polyhistidine-Tagged Proteins.
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    Chapter 15 Purification of Antibodies Using Affinity Chromatography.
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    Chapter 16 Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens.
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    Chapter 17 Measuring Protein-Protein Interactions Using Biacore.
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    Chapter 18 Hydrophobic Interaction Chromatography.
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    Chapter 19 Fast Protein Liquid Chromatography.
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    Chapter 20 Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems.
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    Chapter 21 Strategies for the Purification of Membrane Proteins.
  23. Altmetric Badge
    Chapter 22 Antimicrobial Peptide Production and Purification.
  24. Altmetric Badge
    Chapter 23 Lectin Affinity Chromatography (LAC).
Attention for Chapter 20: Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems.
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Chapter title
Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems.
Chapter number 20
Book title
Protein Chromatography
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6412-3_20
Pubmed ID
Book ISBNs
978-1-4939-6410-9, 978-1-4939-6412-3
Authors

Michael Henry, Paula Meleady

Editors

Dermot Walls, Sinéad T. Loughran

Abstract

Liquid chromatography-mass spectrometry (LC-MS) has become a routine powerful technology in clinical proteomic studies for protein identification, protein characterization and the discovery of biomarkers. In this chapter, we describe two protocol methods to analyze clinical patient samples using a resin based depletion column followed by either protein In-gel enzymatic digestion or protein in-solution enzymatic digestion and then analysis by one-dimensional reverse-phase chromatography or two-dimensional strong cation exchange (SCX)-reverse-phase chromatography (RPC).

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 17%
Researcher 1 17%
Student > Postgraduate 1 17%
Student > Master 1 17%
Unknown 2 33%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 1 17%
Biochemistry, Genetics and Molecular Biology 1 17%
Agricultural and Biological Sciences 1 17%
Medicine and Dentistry 1 17%
Unknown 2 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2018.
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#20,346,264
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Outputs from Methods in molecular biology
#9,921
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#355,303
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Outputs of similar age from Methods in molecular biology
#845
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