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Mendeley readers
| Chapter title |
Purification of Polyhistidine-Tagged Proteins.
|
|---|---|
| Chapter number | 14 |
| Book title |
Protein Chromatography
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6412-3_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6410-9, 978-1-4939-6412-3
|
| Authors |
Sinéad T. Loughran, Ronan T. Bree, Dermot Walls |
| Editors |
Dermot Walls, Sinéad T. Loughran |
| Abstract |
His-tagging is the most widespread and versatile strategy used to purify recombinant proteins for biochemical and structural studies. Recombinant DNA methods are first used to engineer the addition of a short tract of poly-histidine tag (His-tag) to the N-terminus or C-terminus of a target protein. The His-tag is then exploited to enable purification of the "tagged" protein by Immobilized Metal Affinity Chromatography (IMAC). Here, we describe efficient procedures for the isolation of highly purified His-tagged target proteins from an E. coli host using IMAC. |
Mendeley readers
The data shown below were compiled from readership statistics for 120 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 120 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 36 | 30% |
| Student > Ph. D. Student | 12 | 10% |
| Other | 8 | 7% |
| Student > Master | 8 | 7% |
| Professor | 3 | 3% |
| Other | 11 | 9% |
| Unknown | 42 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 41 | 34% |
| Agricultural and Biological Sciences | 14 | 12% |
| Engineering | 5 | 4% |
| Chemistry | 4 | 3% |
| Neuroscience | 3 | 3% |
| Other | 8 | 7% |
| Unknown | 45 | 38% |