↓ Skip to main content

Isolation of Plant Organelles and Structures

Overview of attention for book
Cover of 'Isolation of Plant Organelles and Structures'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Isolation of Plant Organelles and Structures in the Post-genomic Era.
  3. Altmetric Badge
    Chapter 2 Isolation of Plant Organelles and Structures
  4. Altmetric Badge
    Chapter 3 Isolation of Nuclei and Nucleoli.
  5. Altmetric Badge
    Chapter 4 Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts.
  6. Altmetric Badge
    Chapter 5 Isolation of Chromoplasts and Suborganellar Compartments from Tomato and Bell Pepper Fruit.
  7. Altmetric Badge
    Chapter 6 Leucoplast Isolation and Subfractionation.
  8. Altmetric Badge
    Chapter 7 Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes.
  9. Altmetric Badge
    Chapter 8 Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane.
  10. Altmetric Badge
    Chapter 9 Isolation of Vacuoles and the Tonoplast.
  11. Altmetric Badge
    Chapter 10 Isolation of Endoplasmic Reticulum and Its Membrane.
  12. Altmetric Badge
    Chapter 11 Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings.
  13. Altmetric Badge
    Chapter 12 Isolation of Autolysosomes from Tobacco BY-2 Cells.
  14. Altmetric Badge
    Chapter 13 Isolation of Protein Storage Vacuoles and Their Membranes.
  15. Altmetric Badge
    Chapter 14 Isolation of the Cell Wall.
  16. Altmetric Badge
    Chapter 15 Isolation of Plasmodesmata.
  17. Altmetric Badge
    Chapter 16 Isolation of Plasma Membrane and Plasma Membrane Microdomains.
  18. Altmetric Badge
    Chapter 17 Enrichment of the Plant Cytosolic Fraction.
  19. Altmetric Badge
    Chapter 18 Isolation of Apoplast.
  20. Altmetric Badge
    Chapter 19 Isolation of Cytosolic Ribosomes.
  21. Altmetric Badge
    Chapter 20 Isolation of Plastid Ribosomes.
  22. Altmetric Badge
    Chapter 21 Isolation of Mitochondrial Ribosomes.
  23. Altmetric Badge
    Chapter 22 Isolation of Microtubules and Microtubule-Associated Proteins.
  24. Altmetric Badge
    Chapter 23 Isolation of Actin and Actin-Binding Proteins.
  25. Altmetric Badge
    Chapter 24 Purification of 26S Proteasomes and Their Subcomplexes from Plants.
Attention for Chapter 11: Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings.
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
2 X users
facebook
1 Facebook page

Readers on

mendeley
4 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings.
Chapter number 11
Book title
Isolation of Plant Organelles and Structures
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6533-5_11
Pubmed ID
Book ISBNs
978-1-4939-6531-1, 978-1-4939-6533-5
Authors

Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, Joshua L. Heazlewood, Zeng, Wei, Ebert, Berit, Parsons, Harriet T, Rautengarten, Carsten, Bacic, Antony, Heazlewood, Joshua L, Parsons, Harriet T., Heazlewood, Joshua L.

Editors

Nicolas L. Taylor, A. Harvey Millar

Abstract

The Golgi apparatus is an essential component in the plant secretory pathway. The enrichment of Golgi membranes from plant tissue is fundamental to the study of this structurally complex organelle. The utilization of density centrifugation for the enrichment of Golgi membranes is still the most widely employed isolation technique. Generally, the procedure requires optimization depending on the plant tissue being employed. Here we provide a detailed enrichment procedure that has previously been used to characterize cell wall biosynthetic complexes from wheat seedlings. We also outline several downstream analyses procedures, including nucleoside diphosphatase assays, immunoblotting, and finally localization of putative Golgi proteins by fluorescent tags.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 25%
Student > Bachelor 1 25%
Researcher 1 25%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Biochemistry, Genetics and Molecular Biology 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 January 2018.
All research outputs
#15,387,502
of 22,893,031 outputs
Outputs from Methods in molecular biology
#5,354
of 13,134 outputs
Outputs of similar age
#256,305
of 420,414 outputs
Outputs of similar age from Methods in molecular biology
#465
of 1,074 outputs
Altmetric has tracked 22,893,031 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,134 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,414 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.