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Mendeley readers
Chapter title |
Isolation of Protein Storage Vacuoles and Their Membranes.
|
---|---|
Chapter number | 13 |
Book title |
Isolation of Plant Organelles and Structures
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6533-5_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6531-1, 978-1-4939-6533-5
|
Authors |
Tomoo Shimada, Ikuko Hara-Nishimura |
Editors |
Nicolas L. Taylor, A. Harvey Millar |
Abstract |
Protein-storage vacuoles (PSVs) are specialized vacuoles that sequester large amounts of storage proteins. During seed development, PSVs are formed de novo and/or from preexisting lytic vacuoles. Seed PSVs can be subdivided into four distinct compartments: membrane, globoid, matrix, and crystalloid. In this chapter, we introduce easy methods for isolation of PSVs and their membranes from pumpkin seeds. These methods facilitate the identification and characterization of PSV components. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Student > Postgraduate | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Medicine and Dentistry | 1 | 33% |
Unknown | 1 | 33% |