You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Isolation of Endoplasmic Reticulum and Its Membrane.
|
|---|---|
| Chapter number | 10 |
| Book title |
Isolation of Plant Organelles and Structures
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6533-5_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6531-1, 978-1-4939-6533-5
|
| Authors |
G. Eric Schaller |
| Editors |
Nicolas L. Taylor, A. Harvey Millar |
| Abstract |
The association of ribosomes with the rough endoplasmic reticulum (ER) is dependent on Mg(2+). The ribosomes can be stripped from the ER by removal of Mg(2+) from the medium, resulting in a reduction in the ER membrane density and a diagnostic shift in migration when ER vesicles are analyzed by equilibrium density gradient centrifugation. Here, I describe the isolation of microsomes from Arabidopsis, followed by the use of the density shift approach in conjunction with equilibrium density gradient centrifugation as a means to diagnose whether a protein is associated with the ER. The same approach can also be used as a means to enrich for ER membranes. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 29% |
| Researcher | 2 | 14% |
| Professor | 2 | 14% |
| Student > Bachelor | 1 | 7% |
| Student > Master | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 43% |
| Biochemistry, Genetics and Molecular Biology | 5 | 36% |
| Unknown | 3 | 21% |