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Mendeley readers
Chapter title |
Isolation of Microtubules and Microtubule-Associated Proteins.
|
---|---|
Chapter number | 22 |
Book title |
Isolation of Plant Organelles and Structures
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6533-5_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6531-1, 978-1-4939-6533-5
|
Authors |
Takahiro Hamada, Seiji Sonobe |
Editors |
Nicolas L. Taylor, A. Harvey Millar |
Abstract |
Microtubules are essential cellular structures in plant cells. They are polymerized from tubulin dimers and are regulated by microtubule-associated proteins (MAPs). Here, we describe a protocol for purifying tubulin dimers and MAPs from plant cells. The protocol involves preparing vacuole-free mini-protoplasts, a high quality cytoplasmic extract, cycles of microtubule polymerization and depolymerization to increase tubulin and MAP concentration, separation of tubulin and MAPs by column chromatography. We also present tubulin purification methods for biochemical assays. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Student > Bachelor | 1 | 17% |
Researcher | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 33% |
Computer Science | 1 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Unknown | 2 | 33% |