Chapter title |
Isolation of the Cell Wall.
|
---|---|
Chapter number | 14 |
Book title |
Isolation of Plant Organelles and Structures
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6533-5_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6531-1, 978-1-4939-6533-5
|
Authors |
Hervé Canut, Cécile Albenne, Elisabeth Jamet |
Editors |
Nicolas L. Taylor, A. Harvey Millar |
Abstract |
This chapter describes a method allowing the purification of the cell wall for studying both polysaccharides and proteins. The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %). At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %). Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification. It plays critical roles during plant development and in response to environmental constraints. It is largely used in the food and textile industries as well as for the production of bioenergy. All these characteristics and uses explain why its study as a true cell compartment is of high interest. The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material. Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 38% |
Researcher | 4 | 25% |
Student > Doctoral Student | 2 | 13% |
Student > Master | 2 | 13% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 31% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Computer Science | 1 | 6% |
Chemical Engineering | 1 | 6% |
Unknown | 7 | 44% |