| Chapter title |
Isolation of Chromoplasts and Suborganellar Compartments from Tomato and Bell Pepper Fruit.
|
|---|---|
| Chapter number | 5 |
| Book title |
Isolation of Plant Organelles and Structures
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6533-5_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6531-1, 978-1-4939-6533-5
|
| Authors |
Cristina Barsan, Marcel Kuntz, Jean-Claude Pech |
| Editors |
Nicolas L. Taylor, A. Harvey Millar |
| Abstract |
Tomato is a model for fruit development and ripening. The isolation of intact plastids from this organism is therefore important for metabolic and proteomic analyses. Pepper, a species from the same family, is also of interest since it allows isolation of intact chromoplasts in large amounts. Here, we provide a detailed protocol for the isolation of tomato plastids at three fruit developmental stages, namely, nascent chromoplasts from the mature green stage, chromoplasts from an intermediate stage, and fully differentiated red chromoplasts. The method relies on sucrose density gradient centrifugations. It yields high purity organelles suitable for proteome analyses. Enzymatic and microscopy assays are summarized to assess purity and intactness. A method is also described for subfractionation of pepper chromoplast lipoprotein structures. |
Mendeley readers
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|---|---|---|
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Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Student > Ph. D. Student | 2 | 25% |
| Student > Doctoral Student | 1 | 13% |
| Other | 1 | 13% |
| Student > Master | 1 | 13% |
| Other | 0 | 0% |
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| Unknown | 1 | 13% |