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Isolation of Plant Organelles and Structures

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Cover of 'Isolation of Plant Organelles and Structures'

Table of Contents

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    Book Overview
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    Chapter 1 The Isolation of Plant Organelles and Structures in the Post-genomic Era.
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    Chapter 2 Isolation of Plant Organelles and Structures
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    Chapter 3 Isolation of Nuclei and Nucleoli.
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    Chapter 4 Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts.
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    Chapter 5 Isolation of Chromoplasts and Suborganellar Compartments from Tomato and Bell Pepper Fruit.
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    Chapter 6 Leucoplast Isolation and Subfractionation.
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    Chapter 7 Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes.
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    Chapter 8 Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane.
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    Chapter 9 Isolation of Vacuoles and the Tonoplast.
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    Chapter 10 Isolation of Endoplasmic Reticulum and Its Membrane.
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    Chapter 11 Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings.
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    Chapter 12 Isolation of Autolysosomes from Tobacco BY-2 Cells.
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    Chapter 13 Isolation of Protein Storage Vacuoles and Their Membranes.
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    Chapter 14 Isolation of the Cell Wall.
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    Chapter 15 Isolation of Plasmodesmata.
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    Chapter 16 Isolation of Plasma Membrane and Plasma Membrane Microdomains.
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    Chapter 17 Enrichment of the Plant Cytosolic Fraction.
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    Chapter 18 Isolation of Apoplast.
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    Chapter 19 Isolation of Cytosolic Ribosomes.
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    Chapter 20 Isolation of Plastid Ribosomes.
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    Chapter 21 Isolation of Mitochondrial Ribosomes.
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    Chapter 22 Isolation of Microtubules and Microtubule-Associated Proteins.
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    Chapter 23 Isolation of Actin and Actin-Binding Proteins.
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    Chapter 24 Purification of 26S Proteasomes and Their Subcomplexes from Plants.
Attention for Chapter 16: Isolation of Plasma Membrane and Plasma Membrane Microdomains.
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Chapter title
Isolation of Plasma Membrane and Plasma Membrane Microdomains.
Chapter number 16
Book title
Isolation of Plant Organelles and Structures
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6533-5_16
Pubmed ID
Book ISBNs
978-1-4939-6531-1, 978-1-4939-6533-5
Authors

Anzu Minami, Daisuke Takahashi, Yukio Kawamura, Matsuo Uemura

Editors

Nicolas L. Taylor, A. Harvey Millar

Abstract

The plasma membrane surrounds the cytoplasm of a cell and functions as a barrier to separate the intracellular compartment from the extracellular environment. Protein and lipid components distribute nonuniformly and the components form clusters with various functions in the plasma membrane. These clusters are called as "microdomains." In plant cells, microdomains have been studied extensively because they play important roles in biotic/abiotic stress responses, cellular trafficking, and cell wall metabolism. Here we describe a standard protocol for the isolation of the plasma membrane and microdomains from plant cells, Arabidopsis and oat.

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Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 22%
Other 1 11%
Professor 1 11%
Student > Bachelor 1 11%
Researcher 1 11%
Other 1 11%
Unknown 2 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 44%
Biochemistry, Genetics and Molecular Biology 3 33%
Unknown 2 22%