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Mendeley readers
| Chapter title |
A Rapid TALEN Assembly Protocol.
|
|---|---|
| Chapter number | 23 |
| Book title |
Polycomb Group Proteins
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6380-5_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6378-2, 978-1-4939-6380-5
|
| Authors |
Arslan Akmammedov, Tomonori Katsuyama, Renato Paro, Akmammedov, Arslan, Katsuyama, Tomonori, Paro, Renato |
| Editors |
Chiara Lanzuolo, Beatrice Bodega |
| Abstract |
Owing to their modular and highly specific DNA recognition mode, transcription activator-like effector nucleases (TALENs) have been rapidly adopted by the scientific community for the purpose of generating site-specific double-strand breaks (DSBs) on a DNA molecule. A pair of TALENs can be used to produce random insertions or deletions of various lengths via nonhomologous end-joining or together with a homologous donor DNA to induce precise sequence alterations by homologous recombination (HR). Here, we describe a method for TALEN assembly (easyT) and a strategy for genome engineering via HR. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 50% |
| Student > Bachelor | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 50% |
| Unknown | 2 | 50% |