Chapter title |
Polycomb Group Proteins
|
---|---|
Chapter number | 26 |
Book title |
Polycomb Group Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6380-5_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6378-2, 978-1-4939-6380-5
|
Authors |
Katsuyama, Tomonori, Paro, Renato, Tomonori Katsuyama Ph.D., Renato Paro, Tomonori Katsuyama |
Editors |
Chiara Lanzuolo, Beatrice Bodega |
Abstract |
Since Ephrussi and Beadle introduced imaginal disc transplantation to Drosophila research in 1936, the method played an important part towards a better understanding of disc patterning, tissue regeneration, and reprogramming phenomena like transdetermination. Despite increasing usage of high-throughput approaches towards solving biological problems this classical manual method is still in use for studying disc development in a semi-physiological context. Here we describe in detail a protocol and provide recommendations on the procedure in particular for analyzing the regenerative potential of imaginal disks. The steps consist of disc dissection and fragmentation, transplantation into the larval or adult abdomen, and the recovery of implants from the host abdomen. Additionally, we also describe how to make the special transplantation needle from a glass capillary. |
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Student > Ph. D. Student | 1 | 17% |
Researcher | 1 | 17% |
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Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Agricultural and Biological Sciences | 2 | 33% |
Unknown | 2 | 33% |