Chapter title |
Analysis of Endogenous Protein Interactions of Polycomb Group of Proteins in Mouse Embryonic Stem Cells.
|
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Chapter number | 14 |
Book title |
Polycomb Group Proteins
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6380-5_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6378-2, 978-1-4939-6380-5
|
Authors |
Lluis Morey, Luciano Di Croce |
Editors |
Chiara Lanzuolo, Beatrice Bodega |
Abstract |
PRC1 complexes contain four core subunits: Pcgf, Phc, Ring1, and Cbx proteins. Interestingly, mammalian genomes have several paralogues for each subunit, which are differentially expressed depending on the cell type, differentiation program, and cellular stimuli. Therefore, identification and characterization of the specific architecture of different PRC1 complexes during cellular differentiation are essential to better understand the function and recruitment mechanism of PRC1 complexes. In this chapter we describe several methods to study Polycomb architecture, and identification of novel interactors in both pluripotent and differentiating mouse embryonic stem cells. |
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