Chapter title |
Polycomb Group Proteins
|
---|---|
Chapter number | 15 |
Book title |
Polycomb Group Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6380-5_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6378-2, 978-1-4939-6380-5
|
Authors |
Marasca, Federica, Marullo, Fabrizia, Lanzuolo, Chiara, Federica Marasca, Fabrizia Marullo, Chiara Lanzuolo |
Editors |
Chiara Lanzuolo, Beatrice Bodega |
Abstract |
Epigenetic mechanisms modulate and maintain the transcriptional state of the genome acting at various levels on chromatin. Emerging findings suggest that the position in the nuclear space and the cross talk between components of the nuclear architecture play a role in the regulation of epigenetic signatures. We recently described a cross talk between the Polycomb group of proteins (PcG) epigenetic repressors and the nuclear lamina. This interplay is important for the maintenance of transcriptional repression at muscle-specific genes and for the correct timing of muscle differentiation. To investigate the synergism between PcG factors and nuclear architecture we improved a chromatin fractionation protocol with the aim to analyze the PcG nuclear compartmentalization. We thus separated PcG proteins in different fractions depending on their solubility. We surprisingly found a consistent amount of PcG proteins in the matrix-associated fraction. In this chapter we describe the chromatin fractionation procedure, a method that can be used to study the nuclear compartmentalization of Polycomb group of proteins and/or PcG targets in murine and Drosophila cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 25% |
Student > Bachelor | 1 | 8% |
Other | 1 | 8% |
Lecturer > Senior Lecturer | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 33% |
Neuroscience | 2 | 17% |
Agricultural and Biological Sciences | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Unknown | 4 | 33% |