Chapter title |
Polycomb Group Proteins
|
---|---|
Chapter number | 11 |
Book title |
Polycomb Group Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6380-5_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6378-2, 978-1-4939-6380-5
|
Authors |
Arrigoni, Alberto, Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Abrignani, Sergio, Bonnal, Raoul J P, Pagani, Massimiliano, Alberto Arrigoni, Valeria Ranzani, Grazisa Rossetti, Ilaria Panzeri, Sergio Abrignani, Raoul J. P. Bonnal, Massimiliano Pagani |
Editors |
Chiara Lanzuolo, Beatrice Bodega |
Abstract |
RNA-Seq is an approach to transcriptome profiling that uses deep-sequencing technologies to detect and accurately quantify RNA molecules originating from a genome at a given moment in time. In recent years, the advent of RNA-Seq has facilitated genome-wide expression profiling, including the identification of novel and rare transcripts like noncoding RNAs and novel alternative splicing isoforms.Here, we describe the analytical steps required for the identification and characterization of noncoding RNAs starting from RNA-Seq raw samples, with a particular emphasis on long noncoding RNAs (lncRNAs). |
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