| Chapter title |
Polycomb Group Proteins
|
|---|---|
| Chapter number | 9 |
| Book title |
Polycomb Group Proteins
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6380-5_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6378-2, 978-1-4939-6380-5
|
| Authors |
Barnes, Claire, Kanhere, Aditi, Claire Barnes, Aditi Kanhere |
| Editors |
Chiara Lanzuolo, Beatrice Bodega |
| Abstract |
Recent advances in next-generation sequencing have revealed that majority of the human genome is transcribed into long and short RNA (ncRNA) transcripts. Many ncRNAs function by interacting with proteins and forming regulatory complexes. RNA-protein interactions are vital in controlling core cellular processes like transcription and translation. Therefore identifying proteins that interact with ncRNAs is central to deciphering ncRNA functions. Here we describe an RNA-protein pull-down assay, which enables the identification of proteins that interact with an RNA under study. As an example we describe pull-down of proteins interacting with ncRNA XIST, which assists in the recruitment of the polycomb-repressive complex-2 (PRC2) and drives X-chromosomal inactivation. |
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