Chapter title |
Polycomb Group Proteins
|
---|---|
Chapter number | 3 |
Book title |
Polycomb Group Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6380-5_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6378-2, 978-1-4939-6380-5
|
Authors |
Löser, Eva, Latreille, Daniel, Iovino, Nicola, Eva Löser, Daniel Latreille, Nicola Iovino |
Editors |
Chiara Lanzuolo, Beatrice Bodega |
Abstract |
This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions. Formaldehyde-cross-linked chromatin is isolated and antibodies against the target of interest are used to determine whether the target is associated with a specific DNA sequence. This can be performed in spatial and temporal manner and it can provide information about the genome-wide localization of a given protein or histone modification if coupled with deep sequencing technology (ChIP-Seq). |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
France | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 4% |
Unknown | 22 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 26% |
Student > Doctoral Student | 3 | 13% |
Student > Master | 3 | 13% |
Student > Bachelor | 2 | 9% |
Other | 2 | 9% |
Other | 2 | 9% |
Unknown | 5 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 35% |
Chemistry | 3 | 13% |
Agricultural and Biological Sciences | 3 | 13% |
Nursing and Health Professions | 1 | 4% |
Neuroscience | 1 | 4% |
Other | 1 | 4% |
Unknown | 6 | 26% |