| Chapter title |
Chromosome Architecture
|
|---|---|
| Chapter number | 3 |
| Book title |
Chromosome Architecture
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3631-1_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3629-8, 978-1-4939-3631-1
|
| Authors |
Jeremy, Grace, Stevens, James, Lowe, Alan R, Grace Jeremy, James Stevens, Alan R. Lowe, Jeremy, G., Stevens, J., Lowe, Alan R., Jeremy, G, Stevens, J, Lowe, AR |
| Editors |
Mark C. Leake |
| Abstract |
In the eukaryotic cell, a large macromolecular channel, known as the Nuclear Pore Complex (NPC), mediates all molecular transport between the nucleus and cytoplasm. In recent years, single-molecule fluorescence (SMF) imaging has emerged as a powerful tool to study the molecular mechanism of transport through the NPC. More recently, techniques such as single-molecule localization microscopy (SMLM) have enabled the spatial and temporal distribution of cargos, transport receptors and even structural components of the NPC to be determined with nanometre accuracy. In this protocol, we describe a method to study the position and/or motion of individual molecules transiting through the NPC with high spatial and temporal precision. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 36% |
| Student > Bachelor | 1 | 9% |
| Lecturer | 1 | 9% |
| Student > Master | 1 | 9% |
| Unknown | 4 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Agricultural and Biological Sciences | 2 | 18% |
| Neuroscience | 2 | 18% |
| Medicine and Dentistry | 1 | 9% |
| Unknown | 4 | 36% |